thy1 chr2 yfp mice  (Jackson Laboratory)

Journal: The Journal of Headache and Pain

Article Title: Spreading depolarization activates the cGAS–STING pathway and drives cranial nociception: therapeutic potential of STING modulation

doi: 10.1186/s10194-026-02267-5

Figure Lengend Snippet: cGAS-STING pathway modulates susceptibility to SD and periorbital mechanical allodynia. A. Schematic representation of the experimental design. B. Change in SD thresholds of C57BL/6J mice following administration of C-176 (or vehicle) and 2’3’ cGAMP (or vehicle). The lines and error bars represent the median ± interquartile range, with individual data points showing the SD thresholds in mM KCl concentration values for each mouse ( n = 6/group). Triangle symbols indicate male mice, and circle symbols indicate female mice. C. HWT were assessed using the periorbital von Frey test in C57BL/6J mice before and after treatment with C-176 or vehicle ( n = 7/group) D. HWT were assessed using the periorbital von Frey test in C57BL/6J mice before and after treatment with 2’3’ cGAMP or vehicle ( n = 5/group for vehicle; n = 8/group for 2’3’ cGAMP). E. Assessment of HWT following optogenetically induced six SDs in Thy1-ChR2-YFP mice which are treated with 2’3’ cGAMP or vehicle prior to SD induction ( n = 6/group). HWTs were measured at 2 and 24 h after SD. The lines represent the mean ± SEM, and individual data points show withdrawal thresholds in grams (g) per mouse. Triangle symbols indicate male mice, and circle symbols indicate female mice. F. Group averages of HWT data at each time point illustrating differences between 2’3’ cGAMP and vehicle-treated groups ( n = 6/group). Values are presented as mean ± SEM. For statistical comparisons, Mann-Whitney U test was used for SD threshold comparisons due to non-normal data distribution, while paired or unpaired Student’s t test with Welch’s correction and one-way ANOVA was used for other comparisons. ns: p > 0.05, *: p < 0.05, **: p < 0.01, ***: p < 0.001 Panel A was created using BioRender.com

Article Snippet: We used 25–35 gr, 8–16 weeks old female and male Thy1-ChR2-YFP mice (Jackson Laboratories, Bar Harbor, ME) ( n = 68) and wild-type C57BL6/J mice ( n = 64).

Techniques: Concentration Assay, MANN-WHITNEY